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Curiosity and Passion

March 1, 2023 by micro

Curiosity and Passion

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Pursuit of curiosity is the founding ethos of Lindsey Burcham’s lab at UT, and you can feel that curiosity and passion come through when speaking to her about the work she’s done, and the plans she has for her lab. 

Burcham, assistant professor of microbiology, received her PhD at Mississippi State University, and completed her post-doc work at the University of Colorado-Anschutz Medical Campus. A majority of her research at both these institutions focused on host-pathogen interactions between Streptococcus pneumoniae, Group B Streptococcus and human beings. She joined the Department of Microbiology in August 2022, and her growing lab will study these interactions. 

S. pneumoniae is a commensal bacteria of the nasopharynx, but can become pathogenic when transplanted to other parts of the body. It is the source of many infections such as pneumonia, recurring ear infections in children, and even meningitis. Likewise, Group B Streptococcus is a commensal bacteria of the vaginal lumen, but becomes extremely harmful if it passes through the placenta of pregnant persons leading to ascending infections with a variety of harmful consequences: miscarriage, still-birth, or even meningitis or sepsis in the neonate. 

“This lifestyle switch where these commensal organisms exist asymptomatically, causing no harm, and they get into the right host or space and just wreak havoc. How they evolved to do both things is the basis of my interest,” Burcham said. 

The lab uses immortalized epithelial cell lines isolated from the vaginal lumen, the cervix, and an endothelial line from the human blood-brain barrier to observe S. pneumoniae’s ability to invade and thrive in the cell line. This is especially helpful for examining the changes to gene expression in both the cell line and the bacteria itself. 

Mice are used in the model for examining Group B Strep. The lab directly inoculates the mouse’s vaginal lumen to see how the bacteria travels to the cervix or uterus; they also place the bacteria in the blood and observe its ability to cross into the mouse’s blood-brain barrier. 

Beyond simply examining the bacteria’s efficiency in the mice based on controlling their diet. They find that zinc-deficient mice have weakened immune response and are more susceptible to infection. 

As she builds her lab, Burcham plans to foster an environment of collaboration and curiosity. 

“You can teach anyone the science, but I can’t teach you to be curious.” 

It will be exciting to see Burcham’s lab explore opportunistic human pathogens at UT, with implications on maternal, fetal, and pediatric health.

An Unfamiliar Form of Communication

March 1, 2023 by micro

An Unfamiliar Form of Communication

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There are definitely more ways to communicate than using words, and human beings are not the only ones who can communicate with each other. 

Doctoral candidate Bridget O’Banion studies the process of chemotaxis between plant root systems and bacteria, an unfamiliar form of inter-species communication. 

“Plants also have bacteria that live inside of them. These bugs help them to ward off pathogens, help them to absorb nutrients from the soil, and help them to retain water.” 

Where humans can move around and leave an environment that’s potentially harmful, plants simply have to remain in place, making them vulnerable to pathogens and other harmful environmental changes. As a result, plants have to adapt by working with the microbes around them. 

“The plant can continually recruit and repel different microbes from the soil based on what it needs. It can send out different signals into the soil to kill or attract different microbes to live inside its tissue.” 

What do the bacteria get out of this relationship? Well, plants can synthesize their own food, primarily in the form of sugars. Microbes feed on a variety of these plant-made carbon sources and thrive in the niche provided to them by the plant. 

For her projects, O’Banion focuses on plant root systems, and how each part releases a different kind of chemical to attract certain microbes. This is called chemotaxis, and it’s how plants and microbes communicate to form that mutually beneficial symbiosis. 

“My favorite part of the process is generally the independence it’s given me. My PI from the get-go let me pick my questions and pursue my own project. Every day when you’re in the lab, you’re seeing something that’s potentially the first thing anyone has ever observed.” 

Independence certainly defines her experience. O’Banion’s process is unique to the average graduate student, since her mentor and other members of her lab changed institutions in 2020, while she remained at UT. Despite the difficulty this poses, O’Banion says that she’s learned to adapt to switching to a more remote collaboration, and has received support from the other professors in the department. 

“There’s no more swiveling my chair around and chatting with the other grad student in my lab. But the department has been super wonderful in making sure I still have a space where I can still do my work and providing me with any support I need.” 

Communication comes in many different forms. Whether it be our face-to-face conversations, or through Zoom collaborations, Bridget O’Banion plans to continue studying plant-microbe interactions, both commensal and pathogenic.

Saving the Planet with Microbes

March 1, 2023 by micro

Saving the Planet with Microbes

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Kennedi Hambrick is going to be a doctor. Not the clinic-and-patients type, but the saving-the-environment with microbiology type. 

Hailing from Jonesborough, Tennessee, Hambrick first came to UT as an undergraduate student with the goal of one day becoming a medical physician, but ended up discovering her love of working in microbiology research after joining a lab while an undergraduate. 

What is unique about Hambrick is that after completing her bachelor’s degree, she was able to remain at UT to pursue her graduate degree, which is not something many pursuing a PhD get to do. However, she says that staying at UT has helped to make the switch easier. 

“I was actually very thankful to have the opportunity to pursue my PhD at the same institution where I got my bachelor’s degree,” Hambrick said. “The sense of community and support I already had with the microbiology department within UT was great, and it really helped ease my transition.” 

Hambrick is currently in her first year, and joined the lab of Associate Professor Erick Zinser researching the interactions between the cyanobacteria Microcystis aeruginosa and its cyanophage MA-LMM01. M. aeruginosa is naturally found in freshwater lakes. Its NIES-298 strain produces a harmful toxin called microcystin, which can contaminate drinking water, causing health issues for those who drink it. Its cyanophage selectively infects and renders it harmless, helping to offset toxin production. 

“In nature, cyanophages decrease the abundance of cyanobacteria in the environment,” Hambrick said. “We wanted to understand if varying levels of microcystin production had an impact on the infectivity of the cyanophage Ma-LMM01.” 

First, bacteria are incubated at different temperatures. When the host bacteria acclimate to the temperatures, they are infected with their cyanophage. Before and after infection, Hambrick takes toxin samples, cell counts, and DNA samples, which allows for comparisons of host toxin productions at the onset of infection for each temperature. Flow cytometry is used throughout the experiment and infection to account for the quantity of viable host cells in co-culture with the cyanophage. 

Although Hambrick is still early in her graduate studies, it is clear that the work she is doing is laying the groundwork to making a positive impact on the environment. She looks forward to continuing her lab work and hopes to make an impact with her research.

Diverse Applications

March 1, 2023 by micro

Diverse Applications

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Alex Grossman, who recently earned his PhD in microbiology, had originally started his journey in higher education with the goal of studying ecology. After completing the NSF-funded Research Experience for Undergraduates (REU) program in the UT Department of Microbiology, however, he discovered a passion for microbiology. 

“Ninety to 99% of all the biology on planet Earth is microbiology, and I would say that’s where my passion and fascination come from,” Grossman said. 

It was how he decided to come to UT to pursue his PhD, and brought the experience full-circle when he mentored an REU student during his PhD. 

“I got to be, for someone else, the source of inspiration that I had received myself when I was an REU student” he said. “I enjoyed that opportunity to nurture an interest in science.” 

Grossman completed his PhD work in Professor Heidi Goodrich-Blair’s lab, who also serves as head of the microbiology department. 

“In my first year it certainly was intimidating to have my research mentor be the department head. It was a bit more hands-off, but I really appreciated getting to be more independent,” Grossman said. “Despite how busy she was, she always went out of her way to always be there for the students, myself and others included.” 

Grossman researched microbe-host symbiosis, using nematodes as the experimental system. Nematodes were ideal to work with because of how small and simple a host they are. Ultimately, the work led him to discovering and characterizing a novel gram-negative bacterial secretion system (Type-XI secretion system). 

“The system is a mechanism the bacteria have evolved for secreting protein in the environment, either to survive off the host or to interact with the host by binding host antigens, immune factors, and the like.” 

What is special about this secretion system is that it is found in the microbiome of humans and other organisms as well. It is exciting to think that a bacterial system discovered in nematodes has implications in human medicine, and certainly speaks to the diverse applications of microbiology. 

Grossman explains that beyond the molecular and bench work he gets to do in the lab, he quite enjoys the bioinformatic and coding work he gets to do as a part of the analysis. 

“There’s something exhilarating about this experiment that took weeks of work and now there’s an excel sheet with a gigabyte of random numbers and you need to find the story and the logic in it.” 

Grossman was inspired to pursue microbiology because of how vastly relevant it is to so many different processes of life overall. Looking forward to his post-doc work, he is excited to seek out new biology and systems to study, while deepening his passion for bioinformatics and lab work.

A Gut Feeling

March 1, 2023 by micro

A Gut Feeling

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Naomi Gilbert started out as an undergraduate student at James Madison University, where she initially found her passion for environmental microbiology. Inspired by her research mentor there, she decided to come to UT for her PhD.

As a part of Professor Steven Wilhelm’s lab, Gilbert was involved in collecting sampling microbes in the open ocean, then using a combination of molecular and computational tools to characterize what microbes are in the ocean, what they do, and how nutrients impact them.

“In undergrad I did some freshwater cyanobacteria work, but Dr. Wilhelm told me about this project that had to do with marine microbiology, and that’s what got me: the prospect of flying to another country and field sampling the open ocean,” said Gilbert, who specialized in examining the phytoplankton, viruses, and bacteria that infect microbes in the ocean to understand how they impact the ocean’s nutrient cycle, and in turn the global carbon cycle.

“Primary productivity is one of the main mechanisms by which the ocean actually takes up carbon dioxide and exports it to the deep ocean,” Gilbert said. “My contribution was showing that there’s certain nutrients limiting the growth of microbes in the southern ocean, which contribute to primary productivity”

When she first started out, what fascinated Gilbert about the work she was getting to do was the computational analysis and integration of travel – also known as field work, which has extensive preparations.

“The travel might be three weeks total, but months before we’re planning the logistics of all of this,” Gilbert said. “It’s time consuming and tedious, but it was nice to have something to look forward to while you were analyzing your data.”

Gilbert began her undergraduate degree with the goal of pursuing medicine, but shifted gears when she discovered her true passion.

“Not restricting yourself is important. If you get that gut feeling that you want to change course to something you’re more passionate about, it’s better to commit sooner rather than later,” Gilbert said.

Environmental microbiology remains a major interest for Gilbert, and she hopes to continue studying it in her postgraduate work. She thoroughly enjoys the computational analyses she gets to do and hopes to move from the field-based research she is used to into the realm of culture-based lab work.

The Future is Here

March 1, 2023 by micro

The Future is Here

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Dear Friends, Alumni, and Current Members of the UT Department of Microbiology: 

In this edition of our annual newsletter, we focus our attention on what makes our department great: the people of its past, present, and future who comprise a community dedicated to exploring the unseen world and sharing this knowledge with others. 

Many changes occurring within our department and on campus are providing extraordinary opportunities and challenges for our community. We have a new budget model that will change how we prioritize our expenditures and investments in the future. Our College of Arts and Sciences is undergoing structural modifications that will change how we as a department interface with administration and the rest of campus. The Office of Research has announced strategic initiatives that will change how we contextualize our research programs as we seek new funding and develop collaborations with others. 

We are seeing changes in career goals of our students, with an increased emphasis on health-related careers, particularly in infectious disease and immunology. This demand for a health-related curriculum provides us with an opportunity to develop new courses and programs that meet the needs of the future health workforce of the nation. 

Our campus is experiencing dramatic increases in enrollment which will change how we teach effectively. Increased enrollment expands our reach, giving us the opportunity to educate more students about the importance and fascinating features of microbes. It also challenges us to change our pedagogy to enhance learning in large class sizes and to be creative in identifying space and resources to ensure that critical hands-on, modernized instructional lab experiences are available to all registered students. 

Our collective ability to meet all of these challenges and thrive as a department is due to the ongoing effort and creativity of our people. I am pleased to announce the arrival this year of our new assistant professors, Lindsey Burcham and Andrew Monteith. They bring new research and instructional expertise in infectious diseases and immunology, relevant to the new campus Human Health and Wellness initiative. They are launching their independent research programs and building new collaborations within the department, across campus, and beyond. 

Our talented undergraduate instructional team includes Distinguished Lecturer Elizabeth McPherson and Research Assistant Professor Gary LeCleir, as well as new members: Lecturer Jessica Pyle and Research Assistant Professor Zachary Burcham. These extremely talented instructors help us move into the future through their excellence in classroom instruction, by their continuous improvement of the curriculum, and by supporting the graduate student teaching assistants under their supervision. 

Our faculty offer high-demand courses that span the tremendous breadth of microbiology, from ecology, physiology, and molecular biology to virology, immunology, and pathogenesis. In addition, in collaboration with our sister biology departments, we have created a new global health concentration within the biology BS degree. This concentration emphasizes the One Health integration of environmental, agricultural, and human, and public health. 

Graduate students are the thread that weaves through, and binds the department together with their research, teaching, and service. In this edition of the newsletter we present the stories from several graduate students and their past, present and future perspectives. I hope you’ll agree with me that thanks to them, the future is very bright! 

Heidi Goodrich-Blair
Department Head
David and Sandra White Professor of Microbiology